3P6Y
CF Im25-CF Im68-UGUAA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.783, 129.419, 111.159 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.22877 |
| Rwork | 0.226 |
| R-free | 0.27548 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p5t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.978 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 59607 | |
| Completeness [%] | 95.9 | 95.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 283 | 15% PEG 3350, 9% dioxane and 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
