3P6K

Crystal structure of a PLP-dependent aminotransferase (ZP_03625122.1) from Streptococcus suis 89-1591 at 2.07 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97920,0.97903
Spacegroup name	P 32 2 1
Unit cell lengths	83.979, 83.979, 176.391
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.399 - 2.070
R-factor	0.1797
Rwork	0.178
R-free	0.20790
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.794
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.399	29.399	2.140
High resolution limit [Å]	2.070	4.440	2.070
Rmerge	0.052	0.018	0.514
Number of reflections	44694	8500	7951
<I/σ(I)>	15.2	48.9	2
Completeness [%]	99.1	98.7	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.20M lithium sulfate 2.00M ammonium sulfate, Additive: 0.001M pyridoxal 5'-phosphate (PLP), NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K