3P66

Time-dependent and Protein-directed In Situ Growth of Gold Nanoparticles in a Single Crystal of Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	123.2
Detector technology	CCD
Collection date	2009-11-24
Detector	MAR CCD 165 mm
Wavelength(s)	1.0988
Spacegroup name	P 43 21 2
Unit cell lengths	78.543, 78.543, 37.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.360
R-factor	0.2192
Rwork	0.216
R-free	0.24330
Structure solution method	AB INITIO
RMSD bond length	0.009
RMSD bond angle	0.025
Data reduction software	HKL-2000
Phasing software	MOLREP (in the CCP4 Package)
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.310
Rmerge	0.162
Number of reflections	28492
<I/σ(I)>	62.2
Completeness [%]	97.3
Redundancy	27.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	298	6.5% NaCl (w/v) in 0.1 M sodium acetate, pH 4.5, EVAPORATION, temperature 298K