3P66
Time-dependent and Protein-directed In Situ Growth of Gold Nanoparticles in a Single Crystal of Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 123.2 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0988 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.543, 78.543, 37.154 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.360 |
| R-factor | 0.2192 |
| Rwork | 0.216 |
| R-free | 0.24330 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.025 |
| Data reduction software | HKL-2000 |
| Phasing software | MOLREP (in the CCP4 Package) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.310 |
| Rmerge | 0.162 |
| Number of reflections | 28492 |
| <I/σ(I)> | 62.2 |
| Completeness [%] | 97.3 |
| Redundancy | 27.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.5 | 298 | 6.5% NaCl (w/v) in 0.1 M sodium acetate, pH 4.5, EVAPORATION, temperature 298K |
