3P63
Structure of M. laminosus Ferredoxin with a shorter L1,2 loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-06 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.976 |
Spacegroup name | P 41 |
Unit cell lengths | 58.997, 58.997, 55.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.720 - 2.300 |
R-factor | 0.22266 |
Rwork | 0.219 |
R-free | 0.28751 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rfk |
RMSD bond length | 0.015 |
RMSD bond angle | 1.703 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 58.720 |
High resolution limit [Å] | 2.100 |
Number of reflections | 10318 |
Completeness [%] | 97.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 19% PEG 4000, 0.1M Tris buffer pH 7.2, 0.1M-0.18M Mg(NO3)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |