3P5W
Actinidin from Actinidia arguta planch (Sarusashi)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-15 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 2.29 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.734, 56.096, 70.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.930 - 2.200 |
| R-factor | 0.1785 |
| Rwork | 0.172 |
| R-free | 0.23920 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.066 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.071 | 0.216 |
| Number of reflections | 10270 | |
| <I/σ(I)> | 41.2 | 5.1 |
| Completeness [%] | 99.2 | 92.3 |
| Redundancy | 11.9 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 288 | VAPOR DIFFUSION, HANGING DROP , pH 6, temperature 288K |
