3P5N
Structure and mechanism of the S component of a bacterial ECF transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.434, 94.244, 115.402 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.210 - 3.600 |
R-factor | 0.266 |
Rwork | 0.265 |
R-free | 0.28920 |
Structure solution method | MAD |
RMSD bond length | 0.039 |
RMSD bond angle | 1.870 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
Rmerge | 0.065 | 0.029 | 0.831 |
Number of reflections | 6783 | ||
<I/σ(I)> | 9.8 | ||
Completeness [%] | 99.7 | 97.5 | 100 |
Redundancy | 6.9 | 6.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |