3P59
First Crystal Structure of a RNA Nanosquare
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2010-04-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9202752 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 62.378, 62.378, 126.268 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.059 - 2.179 |
R-factor | 0.157 |
Rwork | 0.156 |
R-free | 0.19200 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.114 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.060 | 2.270 |
High resolution limit [Å] | 2.157 | 2.157 |
Rmerge | 0.065 | 0.330 |
Number of reflections | 25640 | |
<I/σ(I)> | 49.5 | 2 |
Completeness [%] | 86.7 | 30 |
Redundancy | 30.2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 150 mM magnesium chloride, 50 mM sodium cacodylate, 10mM hexamminecobalt (III) chloride, 50 mM KCl, 17% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |