3P53
Structure of fascin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795, 0.9798, 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.693, 71.010, 112.736 |
| Unit cell angles | 90.00, 131.23, 90.00 |
Refinement procedure
| Resolution | 30.661 - 2.000 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.22020 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.003 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.462 |
| Number of reflections | 63704 | |
| <I/σ(I)> | 22.5 | 2.6 |
| Completeness [%] | 97.9 | 87.3 |
| Redundancy | 6.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 24% PEG 3350, 0.2 M Lithium Acetate, 1mM DTT, 4% glycerol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 24% PEG 3350, 0.2 M Lithium Acetate, 1mM DTT, 4% glycerol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
