3P4Y

Helicase domain of reverse gyrase from Thermotoga maritima - P2 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-27
Detector	MARRESEARCH
Wavelength(s)	0.984
Spacegroup name	P 1 2 1
Unit cell lengths	61.853, 59.185, 67.650
Unit cell angles	90.00, 98.22, 90.00

Refinement procedure

Resolution	44.344 - 3.200
R-factor	0.2868
Rwork	0.286
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oiy
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	XDS ((VERSION May 10)
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.171	0.612
Number of reflections	7475
<I/σ(I)>	7.2
Completeness [%]	91.4	99.9
Redundancy	3.4	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1