3P4R
Crystal structure of Menaquinol:fumarate oxidoreductase in complex with glutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.678, 138.016, 269.558 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.050 |
R-factor | 0.249 |
Rwork | 0.248 |
R-free | 0.28400 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.111 | 0.084 | 0.394 |
Number of reflections | 88348 | ||
<I/σ(I)> | 20.4 | ||
Completeness [%] | 88.3 | 94.5 | 67.1 |
Redundancy | 2.6 | 3.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.8 | 298 | 14.5% PEG 8000 mme, 125mM MgAc, 95mM Citrate pH 5.8, 0.1% w/v DTT, 0.1mM EDTA, 10mM glutarate, VAPOR DIFFUSION, temperature 298K |