3P4M
Crystal Structure of H2-Kb in complex with the NP205-LCMV epitope YTVKYPNL, an 8-mer peptide from the LCMV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-08-30 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.730, 90.920, 89.050 |
Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
Resolution | 19.987 - 2.500 |
R-factor | 0.1899 |
Rwork | 0.181 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vac |
RMSD bond length | 0.007 |
RMSD bond angle | 1.119 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.600 | |
High resolution limit [Å] | 2.500 | 10.000 | 2.500 |
Rmerge | 0.082 | 0.021 | 0.231 |
Rmeas | 0.026 | 0.288 | |
Number of reflections | 32903 | 427 | 3721 |
<I/σ(I)> | 10.76 | 26.6 | 4.4 |
Completeness [%] | 95.3 | 75.2 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2M ammonium acetate, 16-24% PEG 3350, 0.1M Na-cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 277K |