3P4K
The third conformation of p38a MAP kinase observed in phosphorylated p38a and in solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.641, 82.641, 123.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.780 - 2.304 |
| R-factor | 0.17882 |
| Rwork | 0.176 |
| R-free | 0.23937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lez |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 22102 |
| <I/σ(I)> | 20.7 |
| Completeness [%] | 99.9 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.45M (NH4)2SO4, 0.2M Li2SO4, 0.1M Hepes pH 7.0-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
