3P4C
Alternatingly modified 2'Fluoro RNA octamer f/rA2U2-R32
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.7687 |
Spacegroup name | H 3 2 |
Unit cell lengths | 40.550, 40.550, 117.222 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.150 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.24900 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.027 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.170 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.095 | 0.408 |
Number of reflections | 13343 | |
<I/σ(I)> | 50.1 | 2.43 |
Completeness [%] | 97.9 | 72.9 |
Redundancy | 15.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | Sodium cacodylate (40 mM, pH 6.0), lithium chloride (40 mM), strontium chloride (80 mM), spermine tetrahydrochloride (12 mM), and 2-methyl-2,4-pentanediol (MPD; 10% (v/v)), equilibrated against a reservoir of MPD (1 mL, 35%) , VAPOR DIFFUSION, HANGING DROP, temperature 291K |