3P4A
2'Fluoro modified RNA octamer fA2U2
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.7689 |
Spacegroup name | P 3 |
Unit cell lengths | 43.245, 43.245, 60.791 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.200 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL2Map |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.050 | 0.280 |
Number of reflections | 39940 | |
<I/σ(I)> | 38.8 | 3.4 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 4.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 40mM Sodium cacodylate pH 6.0, 80 mM strontium chloride, 20 mM magnesium chloride, 12mM spermine tetrahydrochloride, 10% 2-methyl-2,4-pentanediol, equilibrated against a reservoir of 35% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 40mM Sodium cacodylate pH 6.0, 80 mM strontium chloride, 20 mM magnesium chloride, 12mM spermine tetrahydrochloride, 10% 2-methyl-2,4-pentanediol, equilibrated against a reservoir of 35% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 291K |