3P3Y
Crystal structure of neurofascin homophilic adhesion complex in space group p6522
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-14 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54981 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 171.425, 171.425, 87.792 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.710 - 2.600 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.28800 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.487 |
| Number of reflections | 23568 | |
| <I/σ(I)> | 19.9 | 2.4 |
| Completeness [%] | 98.8 | 96.4 |
| Redundancy | 5.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 1.4 ammonium sulfate, 0.1 M cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
