3P3K

The crystal structure of translationally controlled tumor protein (TCTP) of Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-03-05
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	61.858, 61.858, 111.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.800 - 2.551
R-factor	0.2169
Rwork	0.216
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1txj
RMSD bond length	0.002
RMSD bond angle	0.589
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.800	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.056	0.182
Number of reflections	7436
<I/σ(I)>		7.6
Completeness [%]	99.0	98.2
Redundancy	4.9	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	21.5 % PEG 4000, 1,6-hexanediol, 50 mM bis-tris methane, 50mM magnesium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K