3P34
Polo-like kinase I Polo-box domain in complex with MQSpTPL phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Collection date | 2009-10-25 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.184, 77.057, 88.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.250 - 1.400 |
R-factor | 0.21254 |
Rwork | 0.212 |
R-free | 0.22958 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1UMW (chain A) |
RMSD bond length | 0.006 |
RMSD bond angle | 1.048 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 58.380 |
High resolution limit [Å] | 1.400 |
Number of reflections | 49803 |
Completeness [%] | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 1.0-2.0M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |