3P31
Crystal structure of the NS1 effector domain from influenza A/Vietnam/1203/2004 (H5N1) virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-26 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.540, 133.500, 55.970 |
| Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
| Resolution | 37.630 - 2.450 |
| R-factor | 0.2005 |
| Rwork | 0.197 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ee9 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.819 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.630 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.068 | 0.443 |
| Number of reflections | 26814 | |
| <I/σ(I)> | 12 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 285 | 0.1M HEPES, 0.2M NaSCN, 6.5 % PEG 3350, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
