3P2I
Structure of an antibiotic related Methyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-03-25 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.541 |
Spacegroup name | P 43 |
Unit cell lengths | 64.857, 64.857, 108.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.400 |
Rwork | 0.205 |
R-free | 0.26560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pb3 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.156 |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.102 | 0.072 | 0.256 |
Number of reflections | 19348 | ||
<I/σ(I)> | 10.8 | ||
Completeness [%] | 96.5 | 97.7 | 76.3 |
Redundancy | 8 | 11 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | pH 5.5 |