3P2E

Structure of an antibiotic related Methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Collection date	2010-06-06
Spacegroup name	P 43
Unit cell lengths	64.985, 64.985, 108.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.680
Rwork	0.197
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pb3
RMSD bond length	0.005
RMSD bond angle	1.225
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.710
High resolution limit [Å]	1.680	4.560	1.680
Rmerge	0.066	0.030	0.433
Number of reflections	51311
<I/σ(I)>	12.7
Completeness [%]	100.0	99.4	100
Redundancy	7.4	7.5	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1