3P26
Crystal structure of S. cerevisiae Hbs1 protein (apo-form), a translational GTPase involved in RNA quality control pathways and interacting with Dom34/Pelota
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 110.631, 110.631, 188.115 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.087 - 2.500 |
| R-factor | 0.2193 |
| Rwork | 0.216 |
| R-free | 0.27100 |
| Structure solution method | Se-SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.087 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 39338 | |
| Completeness [%] | 95.7 | 95.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 100mM Tris-HCl, 12% PEG 6000, 150mM NaCl, pH 8.5, VAPOR DIFFUSION, temperature 293K |
