3P1Z
Crystal structure of the Aperopyrum pernix RNA splicing endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 |
| Unit cell lengths | 135.036, 135.036, 156.246 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.239 - 2.800 |
| R-factor | 0.2632 |
| Rwork | 0.260 |
| R-free | 0.31820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.287 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.062 | 0.033 | 0.778 |
| Number of reflections | 87511 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 99.3 | 99.8 | 95 |
| Redundancy | 6.7 | 8.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.25M ammonium sulfate, 0.1M sodium citrate, 0.9M lithium sulfate, 1mM magnesium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
