3P1Y
Crystal structure of the chimeric Archaeoglobus fulgidus RNA splicing endonuclease with the broadest substrate specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-09 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.770, 104.642, 165.303 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.890 - 2.050 |
R-factor | 0.2498 |
Rwork | 0.247 |
R-free | 0.28670 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
Rmerge | 0.095 | 0.044 | 0.673 |
Number of reflections | 88892 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 99.7 | 99.8 | 97.1 |
Redundancy | 14.1 | 14.2 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 2.2M ammonium sulfate, 0.2M potassium sodium tartrate tetrahydrate, 0.1M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |