3P1V
Crystal structure of a metallo-endopeptidases (BACOVA_00663) from Bacteroides ovatus at 1.93 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97908,0.91837 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.919, 84.963, 139.758 |
| Unit cell angles | 90.00, 99.56, 90.00 |
Refinement procedure
| Resolution | 48.910 - 1.930 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.906 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.098 | 0.636 |
| Number of reflections | 63081 | |
| <I/σ(I)> | 8.05 | 1.8 |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 21.30% polyethylene glycol 3350, 0.30M potassium dihydrogen phosphate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
