3P1J

Crystal structure of human GTPase IMAP family member 2 in the nucleotide-free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-21
Detector	RAYONIX MX-300
Wavelength(s)	0.97895
Spacegroup name	P 65
Unit cell lengths	132.472, 132.472, 84.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.580
R-factor	0.2062
Rwork	0.205
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3LXW
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.670
High resolution limit [Å]	2.580	5.550	2.580
Rmerge	0.077	0.025	0.993
Number of reflections	26603
<I/σ(I)>	12.9
Completeness [%]	100.0	99.9	100
Redundancy	6.1	6.2	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	5-fold molar excess of GDP was added to protein stock solution. 1.4M sodium citrate, 0.1M HEPES, pH 7.6, vapor diffusion, hanging drop, temperature 291K