3P1G

Crystal Structure of the Xenotropic Murine Leukemia Virus-Related Virus (XMRV) RNase H Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Detector technology	CCD
Collection date	2010-09-24
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	37.530, 37.530, 100.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.530 - 1.500
R-factor	0.163
Rwork	0.161
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.995
Data scaling software	d*TREK (9.7L)
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.530	37.530	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.098	0.050	0.771
Number of reflections	22272
<I/σ(I)>	7.8	24.2	2.1
Completeness [%]	99.9	99.8	99.3
Redundancy	6.77	7.36	3.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	291	PEG 1500, Magnesium Chloride, Sodium Citrate, pH 4.7, Vapor Diffusion, Hanging Drop, temperature 291K