3P1A

Structure of human Membrane-associated Tyrosine- and Threonine-specific cdc2-inhibitory kinase MYT1 (PKMYT1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-07-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9245
Spacegroup name	P 21 21 21
Unit cell lengths	45.400, 56.300, 114.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.080 - 1.700
R-factor	0.1522
Rwork	0.150
R-free	0.19495
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2C30 and 3CR0
RMSD bond length	0.015
RMSD bond angle	1.560
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.320	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.087	0.445
Number of reflections	32553
<I/σ(I)>	13.2	3.2
Completeness [%]	98.6	95.3
Redundancy	6.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	20% PEG 3350, 0.2M KSCN, 0.1M Bis-Tris Propane, pH 6.5, 10% Ethylene glycol , VAPOR DIFFUSION, SITTING DROP, temperature 293.15K