3P16

Crystal structure of DNA polymerase III sliding clamp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9790
Spacegroup name	P 1 21 1
Unit cell lengths	88.050, 132.974, 113.777
Unit cell angles	90.00, 109.05, 90.00

Refinement procedure

Resolution	50.000 - 2.890
R-factor	0.26124
Rwork	0.260
R-free	0.28655
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pol
RMSD bond length	0.010
RMSD bond angle	1.373
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.890	6.240	2.900
Rmerge		0.024	0.535
Number of reflections	55132
<I/σ(I)>		33.7	2.1
Completeness [%]	98.2	97	98.7
Redundancy	2.8	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	15%(v/v) PEG 3350, 200mM MgCl2, and 100mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K