3P0V
anti-EGFR/HER3 Fab DL11 alone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-07-30 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9774 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.375, 77.168, 82.664 |
Unit cell angles | 90.00, 99.53, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.850 |
R-factor | 0.22969 |
Rwork | 0.227 |
R-free | 0.28018 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.267 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.850 |
Number of reflections | 20173 |
<I/σ(I)> | 9.2 |
Completeness [%] | 100.0 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | MgCl/PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |