3P0U
Crystal Structure of the ligand binding domain of human testicular receptor 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 129.479, 140.963, 184.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.26001 |
| Rwork | 0.258 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.418 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.077 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.077 | ||
| Number of reflections | 17576 | ||
| <I/σ(I)> | 25.2 | ||
| Completeness [%] | 92.7 | 98.2 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M (NH4)2SO4, 0.1M HEPES, 25% w/v PEG 3,350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
