3P09
Crystal Structure of Beta-Lactamase from Francisella tularensis
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.652, 143.466, 46.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.720 - 1.898 |
R-factor | 0.164 |
Rwork | 0.162 |
R-free | 0.20700 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.024 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 41541 | |
<I/σ(I)> | 8.6 | 2 |
Completeness [%] | 98.4 | 83.3 |
Redundancy | 7.9 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25 % w/v Polyehtlyene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |