3P04
Crystal Structure of the BCR protein from Corynebacterium glutamicum. Northeast Structural Genomics Consortium Target CgR8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97899 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.867, 57.885, 76.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.212 - 2.200 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24300 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.403 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.113 | 0.306 |
Number of reflections | 15202 | |
<I/σ(I)> | 18.7 | 5.9 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 4.8 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under Paraffin oil | 5.1 | 278 | 20% PEG 3350, 0.2 M ammonium citrate, pH 5.1, Microbatch under Paraffin oil, temperature 278K |