3P01
Crystal structure of two-component response regulator from Nostoc sp. PCC 7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.876, 111.876, 112.837 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.650 |
| R-factor | 0.2303 |
| Rwork | 0.228 |
| R-free | 0.26790 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.456 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.087 | 0.545 |
| Number of reflections | 23979 | |
| <I/σ(I)> | 41.8 | 4.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.2M calcium acetate, 0.1M HEPES pH 7.5, 10% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
