3OZZ
Structure of a cytochrome b5 core-swap mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 80 |
Detector technology | IMAGE PLATE |
Collection date | 2007-01-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.669, 30.654, 23.553 |
Unit cell angles | 90.00, 95.65, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1awp |
RMSD bond length | 0.019 |
RMSD bond angle | 1.773 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.152 |
Number of reflections | 8707 | |
<I/σ(I)> | 24.5 | 8.2 |
Completeness [%] | 98.4 | 95.3 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 277 | 30% PEG8000, 0.2M MgAc, 0.1M Pipes, pH 7.0, VAPOR DIFFUSION, temperature 277K |