3OZY

Crystal structure of enolase superfamily member from Bordetella bronchiseptica complexed with Mg and m-Xylarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	I 4
Unit cell lengths	116.769, 116.769, 128.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.250 - 1.299
R-factor	0.1552
Rwork	0.154
R-free	0.17160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ozm
RMSD bond length	0.006
RMSD bond angle	1.103
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	37.250
High resolution limit [Å]	1.299
Number of reflections	208552
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2.8M Sodium Acetate trihydrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K