3OZX
Crystal structure of ABCE1 of Sulfolubus solfataricus (-FeS domain)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.374, 63.656, 81.778 |
| Unit cell angles | 89.15, 84.47, 69.82 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.18946 |
| Rwork | 0.186 |
| R-free | 0.24511 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.664 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0046) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 67949 | |
| Completeness [%] | 91.5 | 93.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M NaCacodylate, pH 6.5, 0.2 M Mg(OAc)2, 20% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
