3OZP
Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 complexed with PUGNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9753 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 108.090, 108.090, 175.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.520 - 2.000 |
R-factor | 0.19908 |
Rwork | 0.199 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o7a |
RMSD bond length | 0.007 |
RMSD bond angle | 1.077 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.520 |
High resolution limit [Å] | 2.000 |
Number of reflections | 72888 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100mM HEPES, 200mM MgCl2, 30% PEG400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |