3OZO
Crystal Structure of insect beta-N-acetyl-D-hexosaminidase OfHex1 complexed with NGT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.040, 108.040, 175.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.670 - 2.000 |
| R-factor | 0.2046 |
| Rwork | 0.205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o7a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.670 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 73100 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100mM HEPES, 200mM MgCl2, 35% PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
