3OZI

Crystal structure of the TIR domain from the flax disease resistance protein L6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Detector technology	CCD
Collection date	2009-09-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953693
Spacegroup name	P 21 21 2
Unit cell lengths	65.941, 102.241, 58.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.296 - 2.300
R-factor	0.1766
Rwork	0.174
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jrn
RMSD bond length	0.008
RMSD bond angle	1.001
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.415	19.531	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.033	0.321
Rmeas		0.036	0.347
Rpim		0.014	0.129
Total number of observations		3978	18309
Number of reflections	18088
<I/σ(I)>	16.7	32.5	6.7
Completeness [%]	99.7	94.9	99.8
Redundancy	7.2	6.5	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	36% PEG 200, 0.1M sodium acetate, pH 5.2, 10mM hexammine cobalt(III) chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K