3OZH
Crystal Structure of Beta-Lactamase/D-alanine Carboxypeptidase from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.069, 75.800, 59.080 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 30.578 - 1.907 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19470 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 28658 | |
| <I/σ(I)> | 11.3 | 3.93 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.16 M Calcium acetate, 0.08 M Sodium cacodylate pH 6.5, 14.4 %w/v PEG 8000, 20 %v/v Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
