3OZ7
Crystal Structure of 3-Phosphopglycerate Kinase of Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Detector technology | CCD |
| Collection date | 2001-06-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 131.386, 131.386, 262.617 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.700 |
| R-factor | 0.23276 |
| Rwork | 0.231 |
| R-free | 0.27367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ltk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.130 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.069 | 0.053 | 0.297 |
| Number of reflections | 35575 | ||
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 94.8 | 99.5 | 71.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 298 | Ammonium Sulfate, Hepes Buffer, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
