3OYO
Crystal structure of hemopexin fold protein CP4 from cow pea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.087, 60.138, 67.574 |
| Unit cell angles | 90.00, 111.18, 90.00 |
Refinement procedure
| Resolution | 26.220 - 2.100 |
| R-factor | 0.20895 |
| Rwork | 0.205 |
| R-free | 0.24648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lp9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.647 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.154 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.105 | 0.322 |
| Number of reflections | 27508 | |
| <I/σ(I)> | 1.13 | |
| Completeness [%] | 97.4 | 95.4 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 50mM Tris-HCl, 30% PEG 8000 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
