3OYL
Crystal structure of the PFV S217H mutant intasome bound to magnesium and the INSTI MK2048
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2010-06-08 |
Detector | ADSC |
Wavelength(s) | 0.98011 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 159.700, 159.700, 123.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.090 - 2.540 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oyb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.424 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.094 | 39.094 | 2.680 |
High resolution limit [Å] | 2.540 | 8.030 | 2.540 |
Rmerge | 0.030 | 0.983 | |
Total number of observations | 8787 | 41464 | |
Number of reflections | 52924 | ||
<I/σ(I)> | 11.7 | 38.9 | 1.7 |
Completeness [%] | 99.7 | 97.2 | 100 |
Redundancy | 5.3 | 4.9 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35 M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50 mM Mes-NaOH, 1mM EDTA, pH 6.5, vapor diffusion, hanging drop, temperature 291K |