3OYC

Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI PICA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 41 21 2
Unit cell lengths	159.960, 159.960, 123.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.800 - 2.660
R-factor	0.2032
Rwork	0.202
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oyb
RMSD bond length	0.011
RMSD bond angle	1.478
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.796	39.165	2.800
High resolution limit [Å]	2.660	8.410	2.660
Rmerge		0.018	0.613
Total number of observations		5254	24482
Number of reflections	46307
<I/σ(I)>	10.4	25.1	2
Completeness [%]	99.6	95	100
Redundancy	3.7	3.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.35 M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50 mM Mes-NaOH, 1mM EDTA, pH 6.5, vapor diffusion, hanging drop, temperature 291K