3OYC
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI PICA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 159.960, 159.960, 123.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.800 - 2.660 |
R-factor | 0.2032 |
Rwork | 0.202 |
R-free | 0.22610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oyb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.478 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.796 | 39.165 | 2.800 |
High resolution limit [Å] | 2.660 | 8.410 | 2.660 |
Rmerge | 0.018 | 0.613 | |
Total number of observations | 5254 | 24482 | |
Number of reflections | 46307 | ||
<I/σ(I)> | 10.4 | 25.1 | 2 |
Completeness [%] | 99.6 | 95 | 100 |
Redundancy | 3.7 | 3.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35 M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50 mM Mes-NaOH, 1mM EDTA, pH 6.5, vapor diffusion, hanging drop, temperature 291K |