3OXE
crystal structure of glycine riboswitch, Mn2+ soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.708, 83.708, 198.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.899 |
| R-factor | 0.20175 |
| Rwork | 0.201 |
| R-free | 0.21902 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3owi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.899 | 2.899 |
| Rmerge | 0.060 | 0.564 |
| Number of reflections | 18565 | |
| <I/σ(I)> | 31.3 | 3 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293.15 | 0.05 M Na-cacodylate, pH 5.1, 0.2 M KCl, 8 % (w/v) PEG8000 and 80 mM magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
