3OXD

Crystal structure of glycine riboswitch with two mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 32 2 1
Unit cell lengths	83.969, 83.969, 199.998
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.999
R-factor	0.1965
Rwork	0.196
R-free	0.21404
Starting model (for MR)	3owi
RMSD bond length	0.005
RMSD bond angle	1.064
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	2.999	2.999
Rmerge	0.070	0.520
Number of reflections	16953
<I/σ(I)>	41.5	5.2
Completeness [%]	99.8	99.9
Redundancy	12.1	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	293.15	0.05 M Na-cacodylate, pH 5.1, 0.2 M KCl, 8 % (w/v) PEG8000 and 80 mM magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K