3OWQ
X-Ray Structure of Lin1025 protein from Listeria innocua, Northeast Structural Genomics Consortium Target LkR164
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97890 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.510, 157.041, 57.277 |
Unit cell angles | 90.00, 98.38, 90.00 |
Refinement procedure
Resolution | 28.332 - 2.606 |
R-factor | 0.2271 |
Rwork | 0.225 |
R-free | 0.25980 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.341 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.600 |
Rmerge | 0.083 |
Number of reflections | 58846 |
<I/σ(I)> | 19.6 |
Completeness [%] | 99.1 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: LiCl 0.1M, PEG1K 22%, MES 0.1M, VAPOR DIFFUSION, HANGING DROP |