3OVW

ENDOGLUCANASE I NATIVE STRUCTURE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU FR-C
Temperature [K]	293
Collection date	1994
Spacegroup name	P 1 21 1
Unit cell lengths	57.420, 81.940, 90.990
Unit cell angles	90.00, 105.97, 90.00

Refinement procedure

Resolution	18.000 - 2.300
Rwork	0.158
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE STRUCTURE OF HUMICOLA INSOLENS EG I
RMSD bond length	0.015
RMSD bond angle	0.039
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.072	0.185
Number of reflections	32773
Completeness [%]	91.0	51 *
Redundancy	2.4	1.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	16 *	20 % PEG 8K, 0.2 M MAGNESIUM CHLORIDE, PH 6.5 FOR 0.1 M MOPS. METHOD: HANGING DROP VAPOR DIFFUSION, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.2 (M)
2	1	reservoir	MOPS	0.1 (M)	pH6.5
3	1	reservoir	PEG8000	10-20 (%(w/v))