3OVK
Crystal structure of an XXA-pro aminopeptidase from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97962 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.086, 116.991, 49.447 |
| Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
| Resolution | 58.520 - 2.000 |
| R-factor | 0.18 |
| Rwork | 0.176 |
| R-free | 0.23900 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.792 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.520 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.136 |
| Number of reflections | 32829 | |
| <I/σ(I)> | 68.192 | 14.9 |
| Completeness [%] | 99.9 | 98.3 |
| Redundancy | 8.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.20M ammonium sulfate, 0.10M tri-sodium citrate pH 5.6, 25% w/v PEG4000, cryoprotectant, inclusion of glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
