3OUX

Structure of beta-catenin with phosphorylated Lef-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97945
Spacegroup name	I 4 2 2
Unit cell lengths	115.560, 115.560, 195.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.110 - 2.400
Rwork	0.199
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m1e
RMSD bond length	0.008
RMSD bond angle	1.071
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.500	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.107	0.615
Number of reflections	26264
<I/σ(I)>	12.2	2.2
Completeness [%]	99.9	99.8
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	296	well solution: 7% PEG 3000, 100 mM sodium citrate. protein solution: 100 micro-M beta-cat-arm, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K